However, these advantages come at the expense of an increased total illumination to the specimen and a decreased axial confinement of the illumination pattern.
Light sheets based on dithered optical lattices improve axial resolution and beam uniformity compared Gaussian beams by using axially structured illumination patterns.
However, when equipped with Gaussian beams, the axial resolving power of a light sheet microscope and the observable field of view are inversely related. Light sheet microscopes reduce phototoxicity and background and improve imaging speed compared to widefield and confocal microscopes.
